PCNA Antibody

SKU: orb1251901

Description

PCNA Antibody

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Drosophila, Gallus, Human, Monkey, Mouse, Porcine, Rat, Yeast, Zebrafish
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: SPM350
• Immunogen: Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.
• Target: PCNA
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Disclaimer: For research use only

Alternative Names

Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

Formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (SPM350)

Western blot testing of A) human HeLa and B) mouse NIH3T3 cell lysates with anti-PCNA antibody (clone SPM350). Predicted molecular weight ~29kDa, routinely observed at 29~36kDa.

Quick Database Links

Gene Symbol

PCNA

UniProt

P12004