PCNA antibody

• Catalog Number: orb345566
• Category: Antibodies
• Description: PCNA antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein.
• Concentration: 0.97mg/mL
• Dilution range: ELISA: 1:2,000 - 1:8,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human PCNA protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: P12004
• NCBI: 33239451
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-PCNA antibody, proliferating cell nucl
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29-36 kDa in size corresponding to PCNA protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts using PCNA antibody