PCNA Antibody

SKU: orb345566

Description

PCNA antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:2,000 - 1:8,000, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29-36 kDa in size corresponding to PCNA protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein.
• Purity: This affinity purified antibody is directed against human PCNA protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.97mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-PCNA antibody, proliferating cell nuclear antigen, cyclin

PAGE-MAP (microsphere affinity proteomics) of Rabbit Anti-PCNA Antibody (orb345566). Antibody array western blot binding of gelfree size separated fractions of multiple lysates (solid lines) and shotgun mass spectroscopy identification (dashed lines) of the target band run in parallel correlate confirming the specificity of this antibody against PCNA.

Western blot using Biorbyt's affinity purified anti-PCNA antibody shows detection of PCNA protein. Lane 1: HEK293 whole cell extracts (p/n orb348669). Lane 2: Jurkat whole cell extracts (p/n orb348674). Approximately 25 µg of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10000 dilution of IRDye® 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Other detection systems will yield similar results.

Quick Database Links

UniProt

P12004

RefSeq:33239451