PCNA Antibody

SKU: orb1925772

Description

PCNA Antibody

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB - 1:2000, IHC-P - 1:100-500, IHC - 1:2000, FC - 1:25
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Molecular Weight: 28769 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Disclaimer: For research use only

All lanes: Anti-PCNA Antibody (C-term) at 1:1000 dilution (upper) Lane 1: HeLa Lane 2: HeLa-Knockout Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution. Predicted band size: 28 kDa

Immunohistochemical analysis of paraffin-embedded Human Ovarian cancer section using Pink1. Diluted at 1:2000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-PCNA Antibody at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining PCNA in human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

UniProt

P12004