PCNA Antibody

SKU: orb1536136

Description

PCNA Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution range: ELISA, IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.01 - 0.03 µg/ml)
• Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Hamster, Human, Mouse, Porcine, Rabbit, Rat
• Application Notes:
  Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 30kD band observed in lysates of cell line HeLa (calculated MW of 28.8kD according to NP_002583.1). Approx. 33kD band was observed in lysates of cell line NIH3T3 and of Mouse and Rat Testis. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al,, J Cell Biol. 2009 Sep 7;186(5):645-54.). Recommended concentration: 0.01-0.03 ug/ml. Enzyme immunoassay: This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with LS-C155050.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-NGNIKLSQTSNVD, from the internal region of the protein sequence according to NP_002583.1.
• Target: PCNA
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

PCNA, Cyclin

PCNA antibody (0.01 ug/ml) staining of NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

PCNA