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Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1). |
| Target | PCNA |
| Molecular Weight | Observed: 36kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of extracts of various cell lines, using PCNA antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat liver using PCNA antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using PCNA antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using PCNA antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using PCNA antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using PCNA antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Protocol Information
PCNA Antibody (orb1261873)
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