PCNA Antibody

• Catalog Number: orb1261873
• Category: Antibodies
• Description: PCNA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 36kDa
• Target: PCNA
• UniProt ID: P12004
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PCNA, MGC8367,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PCNA antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat liver using PCNA antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using PCNA antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using PCNA antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using PCNA antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using PCNA antibody at dilution of 1:100. Blue: DAPI for nuclear staining.