PCNA Antibody

• Catalog Number: orb1248648
• Category: Antibodies
• Description: PCNA Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: EIA, ELISA, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-EAPNQEKVSDYE
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 30 kDa
• Target: PCNA
• UniProt ID: P12004
• NCBI: NP_002583.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PCNA, proliferating cell nuclear antigen, MGC8367,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1). Approx.35kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33kDa in Pig Spleen lysates. (calculated MW of 28.8kDa according to Mouse NP_035175.1, Rat NP_071776.1 and Pig NP_001278854.1).The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al, , J Cell Biol. 2009 Sep 7;186(5):645-54.). Recommended concentration: 0.03-0.1ug/ml.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA both as a capturer and as reported in combination with EB11650. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

orb1248648 (0.05 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248648 (0.03 ug/ml) staining of NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248648 (0.3 ug/ml) staining of Pig Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.