PCM-1 Antibody (C-term)

• Catalog Number: orb1929218
• Category: Antibodies
• Description: PCM-1 Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q15154
• MW: 228544 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB - 1:2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Cell Biology
• Note: For research use only

Flow cytometric analysis of K562 cells using PCM-1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lymph reacted with PCM-1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-PCM-1 Antibody (C-term) at 1:500-2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 229 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.