PCK1 Antibody (C-term)

SKU: orb1928611

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:2000, IHC-P-Leica - 1:250
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD25868
• Immunogen: This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1. Antigen Region: 592-622 aa.
• Target: PCK1 {ECO:0000303|PubMed:8490617, ECO:0000312|HGNC:HGNC:8724}
• Molecular Weight: 69195 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, PCK1, PEPCK1

The anti-PCK1 Pab is used in Western blot to detect PCK1 in mouse liver (Lane 1) and mouse small intestine tissue lysates (Lane 2).

Western blot analysis of PCK1 Antibody (C-term) in rat primary hepatocyte cell line lysates. PCK1 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of PCK1 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Immunohistochemical analysis of paraffin-embedded human kidney tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-PCK1 Antibody (C-term) at 1:2000 dilution. Lane 1: Human liver lysate. Lane 2: Human kidney lysate. Lane 3: NCI-H460 whole cell lysate. Lane 4: Mouse liver lysate. Lane 5: Mouse kidney lysate. Lane 6: Rat kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

PCK1 {ECO:0000303|PubMed:8490617, ECO:0000312|HGNC:HGNC:8724}

UniProt

P35558

RefSeq (Protein):NP_002582.3