PCDH20 Antibody (Center)

• Catalog Number: orb1938046
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PCDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-472 amino acids from the Central region of human PCDH20.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q8N6Y1
• MW: 104919 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB31527
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Protocadherin-20, Protocadherin-13, PCDH20, PCDH13
• Note: For research use only
• NCBI: NP_073754.2

All lanes: Anti-PCDH20 Antibody (Center) at 1:1000 dilution. Lane 1: SK-BR-3 whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lane 4: HL-60 whole cell lysate. Lane 5: C6 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 105 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PCDH20 Antibody (Center) at 1:2000 dilution. Lane 1: SK-BR-3 whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lane 4: HL-60 whole cell lysate. Lane 5: Rat brain whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 105 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of PCDH20 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

PCDH20 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

PCDH20 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PCDH20 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.