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Item 1 of 7
PCCA Antibody (Center)
Catalog Number: orb1937563
| Catalog Number | orb1937563 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB31944 |
| Tested applications | IHC-P, WB |
| Reactivity | Human, Rat |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 80059 Da |
| Target | This PCCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-390 amino acids from the Central region of human PCCA. |
| UniProt ID | P05165 |
| NCBI | NP_001121164.1, NP_000273.2, NP_001171475.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Propionyl-CoA carboxylase alpha chain, mitochondri Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-PCCA Antibody (Center) at 1:2000 dilution. Lane 1: human liver lysate. Lane 2: NIH/3T3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-PCCA Antibody (Center) at 1:2000 dilution. Lane 1: human liver lysate. Lane 2: rat cerebellum lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-PCCA Antibody (Center) at 1:2000 dilution. Lane 1: rat cerebellum lysate. Lane 2: human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-PCCA Antibody (Center) at 1:2000 dilution + human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-PCCA Antibody (Center) at 1:2000 dilution + human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-PCCA Antibody (Center) at 1:2000 dilution + human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
PCCA Antibody (Center) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the PCCA antibody detected the PCCA protein (arrow).
