PCCA Antibody (Center)

SKU: orb1937563

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB31944
• Immunogen: This PCCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-390 amino acids from the Central region of human PCCA. Antigen Region: 362-390 aa.
• Target: PCCA (HGNC:8653)
• Molecular Weight: 80059 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Propionyl-CoA carboxylase alpha chain, mitochondrial, PCCase subunit alpha, Propanoyl-CoA:carbon dioxide ligase subunit alpha, PCCA

All lanes: Anti-PCCA Antibody (Center) at 1:2000 dilution. Lane 1: human liver lysate. Lane 2: NIH/3T3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PCCA Antibody (Center) at 1:2000 dilution. Lane 1: human liver lysate. Lane 2: rat cerebellum lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PCCA Antibody (Center) at 1:2000 dilution. Lane 1: rat cerebellum lysate. Lane 2: human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PCCA Antibody (Center) at 1:2000 dilution + human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PCCA Antibody (Center) at 1:2000 dilution + human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PCCA Antibody (Center) at 1:2000 dilution + human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PCCA Antibody (Center) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the PCCA antibody detected the PCCA protein (arrow).

Quick Database Links

Gene Symbol

PCCA (HGNC:8653)

UniProt

P05165

RefSeq (Protein):NP_001121164.1, NP_001171475.1, NP_000273.2