Paxillin Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1173108
• Category: Antibodies
• Description: Paxillin Recombinant Rabbit Monoclonal Antibody
• Target: PXN
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Tested applications: FC, ICC, WB
• Dilution range: WB=1:500-1:2000, ICC/IF=1:50, Flow-Cyt=1:50-1:100
• Antibody Type: Recombinant Antibody
• Clone Number: E2E8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: A431, 3: U87-MG, 4: PC-3, 5: NIH-3T3, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 65 kDa, Observed MW: 45-70 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173108.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line).

Cell line: NIH-3T3, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173108.