PAX8 Antibody

SKU: orb606932

Description

Recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting points. Titration of the recombinant PAX8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: PAX8/2774R
• Immunogen: A human recombinant fragment (aa 60-261) was used as the immunogen for the recombinant PAX8 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free recombinant PAX8 antibody as confirmation of integrity and purity.

IHC testing of FFPE human thyroid with PAX8 antibody. Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH9, for 10-20 min and allow to cool before testing.

IHC testing of FFPE human kidney with PAX8 antibody. Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH9, for 10-20 min and allow to cool before testing.

Quick Database Links

UniProt

Q06710