You have no items in your shopping cart.
Cart summary
Item 1 of 4
Item 1 of 4
PAX8 Antibody (Center)
Catalog Number: orb1929744
| Catalog Number | orb1929744 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This PAX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-313 amino acids from the Central region of human PAX8. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q06710 |
| MW | 48218 Da |
| Tested applications | FC, IHC-P, WB |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Antibody Type | Primary Antibody |
| Clone Number | RB20218 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Paired box protein Pax-8, PAX8 |
| Note | For research use only |
| NCBI | NP_003457.1, NP_054698.1, NP_039246.1, NP_039247.1 |
Flow cytometric analysis of 293 cells using PAX8 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded mouse kidney reacted with PAX8 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PAX8 Antibody (Center) in HL-60 cell line lysates (35 ug/lane). PAX8 (arrow) was detected using the purified Pab.
All lanes: Anti-PAX8 Antibody (Center) at 1:1000 dilution. Lane 1: SK-OV-3 whole cell lysate. Lane 2: 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Item 1 of 1
