PAX5 / BSAP (Early B-Cell Marker) Antibody

SKU: orb751022

Description

Mouse monoclonal antibody against PAX5 conjugated to Biotin

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: Western Blot (2-4ug/ml); ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Raji, DAUDI or Ramos cells. Human tonsil, spleen or lymph node.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: PAX5/3735
• Immunogen: A recombinant fragment (around aa 235-382) of human PAX5 protein (exact sequence is proprietary)
• Target: PAX5
• Source/Expression System: Bioreactor
• Molecular Weight: 42kDa
• Purification: Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Disclaimer: For research use only

Alternative Names

Anti-B cell lineage specific activator antibody, anti-BSAP antibody, anti-EBB-1 antibody, anti-KLP antibody, anti-Paired box 5 antibody

Analysis of Protein Array containing more than 21000 full-length human proteins using PAX5 Mouse Monoclonal Antibody (PAX5/3735) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human Tonsil stained with PAX5 Mouse Monoclonal Antibody (PAX5/3735).

Formalin-fixed, paraffin-embedded human Tonsil stained with PAX5 Mouse Monoclonal Antibody (PAX5/3735).

SDS-PAGE Analysis Purified PAX5 Mouse Monoclonal Antibody (PAX5/3735). Confirmation of Purity and Integrity of Antibody.

Quick Database Links

Gene Symbol

PAX5

UniProt

Q02548

NCBI Gene ID

5079