PVALB Antibody

SKU: orb1247143

Description

PVALB Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Reactivity: Human, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 14-15kDa band observed in Human (Cerebellum) Brain lysates (calculated MW of 12.1kDa according to NP_002845.1). Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3µg/mL. Approx 10-12kDa band also observed in Rat Skeletal Muscle and in Rat Heart cells transfected with human PVALB, but not in untransfected Rat Heart cells. Data kindly provided by Dr Wang Wang, University of Michigan.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5µg/mL.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-GVDEFSTLVAES
• Target: PVALB
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

parvalbumin, HGNC:9704, PVALB, D22S749, parvalbumin alpha, MGC116759

orb1247143 (0.3 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer).r. Detected by chemiluminescence.

orb1247143 (0.15 ug/ml) staining of adenovirus-mediated gene transfer in Rat Heart cells 1) Untransfected, 2) transient transfection with Human PVALB. 3) Untransfected Rat Skeletal Muscle. Detected by chemiluminescence.

orb1247143 (2.5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247143 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

PVALB

UniProt

P20472

RefSeq (Protein):NP_002845.1