parvalbumin Antibody (FITC)

SKU: orb612707

Description

Mouse monoclonal antibody against Paravalbumin conjugated to FITC

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution Range: WB (1:500); IHC (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:500 dilution of SMC-563 was sufficient for detection of Parvalbumin in 15 ug of Rat Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: C12
• Immunogen: Synthetic peptide from the full length Rat Parvalbumin protein
• Target: parvalbumin
• Molecular Weight: 12 kDa
• Purification: Protein A Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Parvalbumin alpha, PRVA_HUMAN, PVALB

Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.

Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.

Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Undifferentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Parvalbumin Antibody. (D) Hoechst (E) Composite.

Quick Database Links

Gene Symbol

parvalbumin

UniProt

P02625

NCBI Gene ID

25269

RefSeq (Protein):NP_071944.1