Parvalbumin Antibody: Biotin

• Catalog Number: orb612706
• Category: Antibodies
• Description: Mouse monoclonal antibody against Paravalbumin conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C12
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a
• Immunogen: Synthetic peptide from the full length Rat Parvalbumin protein
• Concentration: 1mg/ml
• Dilution range: WB (1:500); IHC (1:100)
• Conjugation: Biotin
• MW: 12 kDa
• Target: parvalbumin
• Entrez: 25269
• UniProt ID: P02625
• NCBI: NP_071944.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Parvalbumin alpha antibody, PRVA_HUMAN antibody, P
• Note: For research use only
• Application notes: A 1:500 dilution of SMC-563 was sufficient for detection of Parvalbumin in 15 ug of Rat Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.

Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.

Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Undifferentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Parvalbumin Antibody. (D) Hoechst (E) Composite.