Parvalbumin Antibody: APC

• Catalog Number: orb612704
• Category: Antibodies
• Description: Mouse monoclonal antibody against Paravalbumin conjugated to APC
• Target: parvalbumin
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: APC
• Reactivity: Human, Mouse, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Purification: Protein A Purified
• Immunogen: Synthetic peptide from the full length Rat Parvalbumin protein
• UniProt ID: P02625
• MW: 12 kDa
• Tested applications: ICC, IF, IHC
• Dilution range: WB (1:500); IHC (1:100)
• Application notes: A 1:500 dilution of SMC-563 was sufficient for detection of Parvalbumin in 15 ug of Rat Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: C12
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Parvalbumin alpha antibody, PRVA_HUMAN antibody, P
• Research Area: Neuroscience, Signal Transduction
• Note: For research use only
• Entrez: 25269
• NCBI: NP_071944.1
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.

Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.

Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Undifferentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Parvalbumin Antibody. (D) Hoechst (E) Composite.