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PARP1 Antibody (N-term)
SKU: orb1429861
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Description
Research Area
Cell Biology, Metabolism Research
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IF, WB |
|---|---|
| Dilution Range | WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB14153 |
| Immunogen | Synthetic Peptide |
| Target | PARP1 |
| Molecular Weight | 113084 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, PARP1, ADPRT, PPOL
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PARP1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PARP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the PARP1 antibody detected the PARP1 protein (arrow).
Western blot analysis of PARP1 (arrow) using rabbit polyclonal PARP1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (Lane 2).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001609.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
PARP1 Antibody (N-term) (orb1429861)
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