PARP1 Antibody

SKU: orb1274565

Description

PARP1 Antibody

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:1000 - 1:3000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200CHIP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).
• Target: PARP1
• Molecular Weight: Observed: 100kDa, 110kDa, 125kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KIF3B, PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1

Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:500 dilution.

Western blot analysis of extracts of Jurkat cells, using PARP1 antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using PARP1 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using PARP1 antibody at dilution of 1:200 (40x lens).

Quick Database Links

Gene Symbol

PARP1

UniProt

P09874