PARK7 Antibody

• Catalog Number: orb1247145
• Category: Antibodies
• Description: PARK7 Antibody
• Target: PARK7
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-AAQVKAPLVLKD
• UniProt ID: Q99497
• Tested applications: ELISA, IF, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24kDa band observed in lysates of cell lines HeLa and Jurkat and of Human Brain (Cerebellum, Hippocampus, Frontal Cortex) and rodent Brain lysates (calculated MW of 19.9kDa according to NP_001116849.1 and of NP_009193.2). Recommended concentration: 0.05-0.1ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen around the nucleus. Dividing cells appear to show enhanced expression. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PARK7, DJ1, DJ-1, Parkinson disease (autosomal rec
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_009193.2

orb1247145 (0.001 ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247145 (0.001 ug/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5 ug/ml orb1247145 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.

orb1247145 (3.75 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247145 (3.75 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.