Pard3b Antibody

SKU: orb1248791

Description

Pard3b Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 125kDa band observed in Mouse fetal Kidney lysates (calculated MW of 133kDa according to NP_001074519.2). Recommended concentration: 0.3-1µg/mL. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RNQDPRQKNP
• Target: Pard3b
• Molecular Weight: Approx 125 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pard3b, par-3 partitioning defective 3 homolog B (C. elegans), DN-276O13.2, 1810008K04Rik, 2010002N16Rik, 2810455B10Rik, Als2cr19, PAR3B, PAR3L, PAR3beta, RP23-20G7.1, DN-276O13.2, PAR3-L protein, PAR3-beta, amyotrophic lateral sclerosis 2 chromosomal region candidate gene 19 protein homolog, partitioning defective 3 homolog B, partitioning defective 3-like protein

orb1248791 (0.5 ug/ml) staining of Mouse fetal Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Pard3b

UniProt

Q9CSB4

RefSeq (Protein):NP_001074519.2