CUL9 Antibody

SKU: orb750503

Description

CUL9 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:1,000
• Reactivity: Human
• Application Notes:
  Anti-PARC reacts with human and mouse PARC by western blot and immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others). To date co-immunoprecipitation using 35S-IP has been negative. A 281.2 kDa band corresponding to human PARC is detected. Most cell lines expressing PARC can be used as a positive control. Researchers should determine optimal titers for other applications.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2503-2517 of Human PARC (C-terminus) coupled to KLH.
• Target: CUL9
• Purity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse PARC. Cross reactivity with PARC from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-PARC Antibody, p53 associated parkin like cytoplasmic protein antibody, RP3 330M21.2 antibody, UbcH 7 associated protein 1 antibody

Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF (SKP1/cullin-1/F-box protein) / CBC (cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.

Quick Database Links

Gene Symbol

CUL9

UniProt

Q8IWT3

RefSeq:Q8IWT3.2