PAPPA2/Pappalysin 2 Antibody

SKU: orb1538546

Description

PAPPA2/Pappalysin 2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Dilution Range: ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Further information: PLAC3 antibody can be used for detection of PLAC3 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A 15 AA synthetic peptide near the N-Terminus of human PLAC3. The immunogen is located within aa 450-500 of PLAC3.
• Target: PAPPA2 / Pappalysin 2
• Purification: Peptide Column Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PAPPA2, PAPP-A2, PAPP-E, PLAC3, Pappalysin-2, PAPPE, Placenta-specific 3, Pappalysin 2

Immunocytochemistry of PLAC3 in HeLa cells with PLAC3 antibody at 10 ug/ml.

Immunofluorescence of PLAC3 in HeLa cells with PLAC3 antibody at 20 ug/ml.

Western blot analysis of PLAC3 in HeLa cell lysate with PLAC3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Quick Database Links

Gene Symbol

PAPPA2 / Pappalysin 2