PAPP-A Antibody / Pappalysin-1

SKU: orb2642937

Description

Pregnancy Associated Plasma Protein (PAPP-A) is found in maternal blood that increases as pregnancy progresses, although it is not specific to pregnancy. It is principally expressed in the syncytiotrophoblast of the placenta, which forms the main source of circulating maternal PAPP-A. It cleaves insulin-like growth factor binding proteins (IGFBPs), IGFBP-4 and IGFBP-5. IGFBP-4 cleavage is enhanced significantly in the presence of bound IGF, whereas IGFBP-5 cleavage is inhibited slightly by IGF presence. It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. PAPPA has also been suggested as a potential biomarker of acute myocardial infarction and Coronary Artery Disease (CAD).

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Reactivity: Human
• Application Notes:
  Optimal dilution of the PAPP-A antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: PAPPA/2718
• Immunogen: A portion of amino acids 351-523 from the human protein was used as the immunogen for the PAPP-A antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human placenta with PAPP-A antibody (clone PAPPA/2718). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human placenta with PAPP-A antibody (clone PAPPA/2718). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human placenta with PAPP-A antibody (clone PAPPA/2718). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PAPP-A antibody (clone PAPPA/2718) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q13219