PAPP-A antibody

SKU: orb2276383

Description

PAPP-A antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:75-1:150
• Reactivity: Human
• Application Notes:
  Positive Control: Placenta: Trophoplast and chorion cells should show a strong cytoplasmic papp-A staining. Negative Control: Colon: Papp-A immunostaining should be completely absent in both stromal end epithelial cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit according to the manufacturer’s directions.

  Read more...

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: MSVA-780M
• Immunogen: Recombinant fragment (within aa 351-523) of human PAPP-A protein (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASBABP2; Aspecific BCL2 ARE binding protein 2; Differentially placenta 1 expressed protein; DIPLA1; IGF-dependent; IGFBP4ase; Insulin-like growth factor-dependent IGF-binding protein 4 protease (IGFBP-4 protease); PAPA; PAPP A; PAPPA1; Pappalysin-1; Pregnancy Associated Plasma Protein A (PAPP-A)

Quick Database Links

UniProt

Q13219