Pan-HLA Antibody MHC II (DP/DQ/DR)

SKU: orb1151846

Description

Mouse monoclonal antibody to Pan-HLA MHC II (DP/DQ/DR)

Images & Validation

• Tested Applications: FACS, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Pan-HLA antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: HLA-Pan/6663
• Immunogen: Priess human B cell line was used as the immunogen for the Pan-HLA antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human tonsil tissue with Pan-HLA antibody (clone HLA-Pan/6663). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with Pan-HLA antibody (clone HLA-Pan/6663). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P04440, P01920, P01908, P01909, P01903