Pan-HLA Antibody MHC II (DP/DQ/DR)

• Catalog Number: orb1151846
• Category: Antibodies
• Description: Mouse monoclonal antibody to Pan-HLA MHC II (DP/DQ/DR)
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Priess human B cell line was used as the immunogen for the Pan-HLA antibody.
• UniProt ID: P01903, P01909, P01920, P04440, P01908
• Tested applications: FACS, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Pan-HLA antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: HLA-Pan/6663
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Pan-HLA antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil tissue with Pan-HLA antibody (clone HLA-Pan/6663). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with Pan-HLA antibody (clone HLA-Pan/6663). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.