pan-Filovirus GP/Envelope glycoprotein Antibody

SKU: orb3152801

Description

pan-Filovirus GP/Envelope glycoprotein Antibody is an unconjugated antibody. It shows reactivity with Virus samples. It is suitable for use in ELISA and WB applications. The antibody is supplied in liquid form. Purity is >95% as determined by SDS-PAGE.

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Virus

Key Properties

• Antibody Type: Primary Antibody
• Host: Human
• Clone No.: BO43F32
• Purity: >95% as determined by SDS-PAGE.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RecName: Full=Envelope glycoprotein; AltName: Full=GP1,2; Short=GP; Contains: RecName: Full=Shed GP; AltName: Full=GP1,2-delta; Contains: RecName: Full=GP1; Contains: RecName: Full=GP2; Flags: Precursor, GP, GP1,2, /evidence=ECO:0000255., Filovirus glycoprotein; pfam01611, Envelope glycoprotein. /id=PRO_0000037485., Extracellular. /evidence=ECO:0000255., Shed GP. /id=PRO_0000445686., GP1. /id=PRO_0000037486., /evidence=ECO:0007829|PDB:6HS4., N->D: Induces GP1 secretion. Complete loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., Interchain (between GP1 and GP2 chains). /evidence=ECO:0000269|PubMed:12438572., /evidence=ECO:0007829|PDB:6QD7., C->G: Induces GP1 secretion. Complete loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., Receptor-binding. /evidence=ECO:0000255., D->A: 80% loss of virus capability to enter into host cell., D->E,K: No effect on viral entry., Involved in receptor recognition and/or post-binding events. /evidence=ECO:0000255., L->A: Complete loss of virus capability to enter into host cell., L->F,I,K: 90% loss of virus capability to enter into host cell., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., Involved in receptor recognition and/or post-binding events. /evidence=ECO:0000255., L->A: 90% loss of virus capability to enter into host cell., L->F: Almost complete loss of virus capability to enter into host cell., L->K: Complete loss of virus capability to enter into host cell., Involved in receptor recognition and/or post-binding events. /evidence=ECO:0000255., R->A,E: Complete loss of virus capability to enter into host cell., R->K: No loss of virus capability to enter into host cell., S -> P (in strain: Isolate mouse-adapted)., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., F->A,E: Complete loss of virus capability to enter into host cell., F->A: About 50% loss of ability to counteract host BST2. /evidence=ECO:0000269|PubMed:27707924., F->I: No loss of virus capability to enter into host cell., Involved in receptor recognition and/or post-binding events. /evidence=ECO:0000255., Involved in receptor recognition and/or post-binding events. /evidence=ECO:0000255., K->A,E: 80% loss of virus capability to enter into host cell., K->R: 20% loss of virus capability to enter into host cell., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0000255., C->G: Almost complete loss of expression of GP1 and GP2. Almost complete loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., L->A: About 60% loss of ability to counteract host BST2. /evidence=ECO:0000269|PubMed:27707924., /evidence=ECO:0007829|PDB:9BSV., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0000255., C->G: Reduced levels of expression of GP1 and GP2. 50% loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., L->A: About 60% loss of ability to counteract host BST2. /evidence=ECO:0000269|PubMed:27707924., /evidence=ECO:0007829|PDB:6HS4., C->S: Almost complete loss of expression of GP1 and GP2. Complete loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., C->S: Reduced levels of expression of GP1 and GP2. Almost complete loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., I->A: 90% loss of virus capability to enter into host cell., I->E: Complete loss of virus capability to enter into host cell., I->F: 50% loss of virus capability to enter into host cell., Involved in receptor recognition and/or post-binding events. /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:7JPI., /evidence=ECO:0007829|PDB:7JPI., N->D: No effect on GP1 and GP2 expression. No loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:6HS4., N->Y: No effect on GP1 and GP2 expression. 12% loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:6HS4., S -> P (in strain: Isolate mouse-adapted)., /evidence=ECO:0007829|PDB:6HS4., N->D: No effect on GP1 and GP2 expression. 12% loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., N->D: No effect on GP1 and GP2 expression. 18% loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:7TN9., Mucin-like region., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:7LYD., Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite., Polar residues. /evidence=ECO:0000256|SAM:MobiDB-lite., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., invasion associated endopeptidase, N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite., Polar residues. /evidence=ECO:0000256|SAM:MobiDB-lite., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Low complexity. /evidence=ECO:0000256|SAM:MobiDB-lite., Polar residues. /evidence=ECO:0000256|SAM:MobiDB-lite., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., RRTRR->AGTAA: Almost complete loss of cleavage between GP1 and GP2. No loss of infectivity. /evidence=ECO:0000269|PubMed:11152533., RTRR->ATAA: No effect on cleavage between GP1 and GP2. /evidence=ECO:0000269|PubMed:9882347., Cleavage; by host furin. /evidence=ECO:0000269|PubMed:9576958., GP2. /id=PRO_0000037487., /evidence=ECO:0000255., C->G: Induces GP1 secretion. Complete loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:8DPM., Fusion peptide. /evidence=ECO:0000305., /evidence=ECO:0007829|PDB:6HS4., G->R: Reduced infectivity. /evidence=ECO:0000269|PubMed:10482652., L->A,R: Reduced infectivity. /evidence=ECO:0000269|PubMed:10482652., I->A: Reduced infectivity. /evidence=ECO:0000269|PubMed:10482652., I->R: Almost complete loss of infectivity. No effect on transport of GP to the cell surface and incorporation onto virions. /evidence=ECO:0000269|PubMed:10482652., /evidence=ECO:0007829|PDB:6HS4., F->A: Reduced infectivity. /evidence=ECO:0000269|PubMed:10482652., F->R: Almost complete loss of infectivity. No effect on transport of GP to the cell surface and incorporation onto virions. /evidence=ECO:0000269|PubMed:10482652., G->A: Almost complete loss of infectivity. No effect on transport of GP to the cell surface and incorporation onto virions. /evidence=ECO:0000269|PubMed:10482652., P->R: Almost complete loss of infectivity. No effect on transport of GP to the cell surface and incorporation onto virions. /evidence=ECO:0000269|PubMed:10482652., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0007829|PDB:6HS4., I -> T., /evidence=ECO:0007829|PDB:6HS4., /evidence=ECO:0000255., C->S: Induces GP1 secretion. Complete loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., heptad repeat 1-heptad repeat 2 region of the transmembrane subunit of Filoviridae viruses, Ebola virus and Marburg virus, and related domains; cd09850, homotrimer interface [polypeptide binding], HR1-GP1 interface [polypeptide binding], HR1A, /evidence=ECO:0007829|PDB:2EBO., N->D: Reduced levels of expression of GP, GP1 and GP2. 20% loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., HR1B, HR1C, HR1D, Cl binding site [ion binding], immunosuppressive region, /evidence=ECO:0007829|PDB:2EBO., /evidence=ECO:0007829|PDB:2EBO., /evidence=ECO:0000269|PubMed:12438572., CX(6,7)C motif, C->S: Induces GP1 secretion. Complete loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., /evidence=ECO:0007829|PDB:2EBO., C->G: Induces GP1 secretion. Complete loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., C->G: Induces GP1 secretion. Complete loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:2EBO., HR2, N->D: Slightly reduced levels of expression of GP1 and GP2. No loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:12438572., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., D->V: No effect on release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., K->R,V: No effect on release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., T->I: 50% loss of release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., L->V: 60% loss of release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., L->V: Increased GP shedding. /evidence=ECO:0000269|PubMed:26092855., P->A: 60% loss of release of soluble GP1,2delta., Cleavage; by host ADAM17. /evidence=ECO:0000269|PubMed:15103332., D->E: No effect on release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., D->L,V: Increased release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., D->V: Decreased GP shedding. /evidence=ECO:0000269|PubMed:26092855., Q->V: No effect on release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., G->V: 40% loss of release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., D->V: No effect on release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., N->A: No effect on release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., D->V: No effect on release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., N->A: No effect on release of soluble GP1,2delta. /evidence=ECO:0000269|PubMed:15103332., Helical. /evidence=ECO:0000255., Important role for host BST2/tetherin antagonism. /evidence=ECO:0000269|PubMed:27707924., G->L: About 60% loss of viral release; when associated with L-664. /evidence=ECO:0000269|PubMed:29669839., A->L: About 60% loss of viral release; when associated with L-660. /evidence=ECO:0000269|PubMed:29669839., C->A: Reduced palmitoylation. No effect on GP processing and association with retrovirus particle. No loss of virus capability to enter into host cell. Loss of localization to the rafts; when associated with A-670. /evidence=ECO:0000269|PubMed:11152533, ECO:0000269|PubMed:11877482, ECO:0000269|PubMed:12438572., C->F: Slightly reduced levels of expression of GP1 and GP2. Greatly reduced GP processing and association with retrovirus particle. 43% loss of virus capability to enter into host cell. Loss of localization to the rafts; when associated with A-672. /evidence=ECO:0000269|PubMed:11152533, ECO:0000269|PubMed:11877482, ECO:0000269|PubMed:12438572., S-palmitoyl cysteine; by host. /evidence=ECO:0000269|PubMed:11152533., Cytoplasmic. /evidence=ECO:0000255., C->A: Reduced palmitoylation. No effect on GP processing and association with retrovirus particle. No loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:11152533, ECO:0000269|PubMed:12438572., C->F: Slightly reduced levels of expression of GP1 and GP2. Almost no effect on GP processing and association with retrovirus particle. 24% loss of virus capability to enter into host cell. /evidence=ECO:0000269|PubMed:11152533, ECO:0000269|PubMed:12438572., S-palmitoyl cysteine; by host. /evidence=ECO:0000269|PubMed:11152533., Nucleocapsid protein; Protein N, Nucleoprotein. /id=PRO_0000222174., Ebola nucleoprotein; pfam05505, /evidence=ECO:0000255., Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite., Acidic residues. /evidence=ECO:0000256|SAM:MobiDB-lite., Polar residues. /evidence=ECO:0000256|SAM:MobiDB-lite., Acidic residues. /evidence=ECO:0000256|SAM:MobiDB-lite., Polar residues. /evidence=ECO:0000256|SAM:MobiDB-lite., /evidence=ECO:0007829|PDB:6U55., /evidence=ECO:0007829|PDB:6U52., /evidence=ECO:0007829|PDB:6U52., /evidence=ECO:0007829|PDB:6U52., /evidence=ECO:0007829|PDB:6U52., /evidence=ECO:0007829|PDB:6U52., /evidence=ECO:0007829|PDB:6U52., /evidence=ECO:0007829|PDB:6U55., /evidence=ECO:0007829|PDB:6U55., /evidence=ECO:0007829|PDB:6U55., /evidence=ECO:0007829|PDB:6U55., /evidence=ECO:0007829|PDB:6U52., /evidence=ECO:0007829|PDB:6U52., GP1,2; Virion spike glycoprotein, Filovirus glycoprotein; pfam01611, /evidence=ECO:0000250., Envelope glycoprotein. /evidence=ECO:0000250. /id=PRO_0000314986., Extracellular. /evidence=ECO:0000255., GP1. /evidence=ECO:0000250. /id=PRO_0000314987., Receptor-binding. /evidence=ECO:0000250., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:6BP2., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Polar residues. /evidence=ECO:0000256|SAM:MobiDB-lite., Mucin-like region. /evidence=ECO:0000250., Polar residues. /evidence=ECO:0000256|SAM:MobiDB-lite., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Low complexity. /evidence=ECO:0000256|SAM:MobiDB-lite., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Polar residues. /evidence=ECO:0000256|SAM:MobiDB-lite., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Polar residues. /evidence=ECO:0000256|SAM:MobiDB-lite., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Low complexity. /evidence=ECO:0000256|SAM:MobiDB-lite., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Cleavage; by host furin. /evidence=ECO:0000250., GP2. /evidence=ECO:0000250. /id=PRO_0000314988., /evidence=ECO:0007829|PDB:6BP2., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., Fusion peptide. /evidence=ECO:0000250., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., /evidence=ECO:0007829|PDB:6BP2., heptad repeat 1-heptad repeat 2 region of the transmembrane subunit of Filoviridae viruses, Ebola virus and Marburg virus, and related domains; cd09850, homotrimer interface [polypeptide binding], HR1-GP1 interface [polypeptide binding], HR1A, N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., HR1B, HR1C, HR1D, Cl binding site [ion binding], immunosuppressive region, /evidence=ECO:0007829|PDB:6BP2., CX(6,7)C motif, /evidence=ECO:0007829|PDB:6BP2., HR2, N-linked (GlcNAc...) asparagine; by host. /evidence=ECO:0000255., Helical. /evidence=ECO:0000255., Cytoplasmic. /evidence=ECO:0000255., S-palmitoyl cysteine; by host. /evidence=ECO:0000250|UniProtKB:P35253., S-palmitoyl cysteine; by host. /evidence=ECO:0000250|UniProtKB:P35253.

Quick Database Links

UniProt

Q9QP77, Q05320, Q1PDC7