Pan-Cytokeratin, Pan-CK Antibody

SKU: orb1294309

Description

Rabbit polyclonal antibody to pan Cytokeratin

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution range: Western Blot 1:2000–1:5000 Immunofluorescence 1:400–1:800 Immunohistochemistry (Paraffin) 1:400–1:800 Flow Cytometry 1:300–1:500
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Detects endogenous levels of total keratin 5, 6, 7, 8, 14, 17, 18, 19 and 20. The antibody does not cross-react with other keratins.
• Target: Pan-Cytokeratin, Pan-CK
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Anti-pan Cytokeratin antibody - at 1/3000 dilution, Lysates at 45 µg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with orb1294309 overnight at 4°C.

Anti-pan Cytokeratin antibody - at 1/3000 dilution.

Application: Immunocytochemistry (ICC), Sample: CTC (Circulating Tumor Cell), Antibody: Pan-Cytokeratin antibody (orb1294309).

Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: Pan-Cytokeratin antibody (orb1294309).

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294309 anti-pan Cytokeratin0 antibody (red) at 1:300 and 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294309 anti-pan Cytokeratin antibody (green) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling pan Cytokeratin with orb1294309 at 1/400.

pan Cytokeratin antibody, orb1294309, Cell: MCF-7, pan Cytokeratin (orb1294309): 1-350.

Quick Database Links

Gene Symbol

Pan-Cytokeratin, Pan-CK