Pan Cytokeratin Antibody (PE Conjugate)

SKU: orb1716114

Description

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. This MAb is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 5ul per test per one million cells or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100
• Reactivity: Human
• Application Notes:
  Optimal dilution of the PE-conjugated Pan Cytokeratin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: SPM115 + SPM116
• Immunogen: Human epidermal keratin was used as the immunogen for the Pan Cytokeratin antibody.
• Purification: Protein A/G affinity
• Conjugation: PE

Storage & Handling

• Storage: Store the PE-conjugated Pan Cytokeratin antibody in the dark at 2-8°C (do not freeze; conjugate is light sensitive).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q7Z794, Q01546