Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

• Catalog Number: orb1151900
• Category: Antibodies
• Description: Mouse monoclonal antibody to Pan Cytokeratin Cocktail (Acidic + Basic)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM115 + SPM116
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Human epidermal keratin was used as the immunogen for the Pan Cytokeratin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Pan Cytokeratin antibody is available for research use only.
• UniProt ID: Q7Z794, Q01546
• Storage: Aliquot the Pan Cytokeratin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Pan Cytokeratin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human breast carcinoma tissue with Pan Cytokeratin antibody cocktail (clone SPM115 + SPM116). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.