Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

• Catalog Number: orb1151900
• Category: Antibodies
• Description: Mouse monoclonal antibody to Pan Cytokeratin Cocktail (Acidic + Basic)
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Human epidermal keratin was used as the immunogen for the Pan Cytokeratin antibody.
• UniProt ID: Q7Z794, Q01546
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Pan Cytokeratin antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: SPM115 + SPM116
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

IHC staining of FFPE human breast carcinoma tissue with Pan Cytokeratin antibody cocktail (clone SPM115 + SPM116). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.