PAM/Peptidylglycine 2 hydroxylase Polyclonal Antibody

SKU: orb1417816

Description

Rabbit polyclonal antibody to PAM.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Peptidyl-glycine alpha-amidating monooxygenase, PAM, Peptidylglycine alpha-hydroxylating monooxygenase, PHM, 1.14.17.3, Peptidyl-alpha-hydroxyglycine alpha-amidating lyase, 4.3.2.5, Peptidylamidoglycolate lyase, PAL, PAM {ECO:0000303|PubMed:12699694, ECO:0000312|HGNC:HGNC:8596}

Sample: Large intestine (Mouse) Lysate at 40 ug. Primary: Anti- PAM/Peptidylglycine 2 hydroxylase at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 108 kD. Observed band size: 108 kD.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PAM Peptidylglycine 2 hydroxylase) Polyclonal Antibody, Unconjugated at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PAM Peptidylglycine 2 hydroxylase) Polyclonal Antibody, Unconjugated at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Quick Database Links

UniProt

P19021