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Catalog Number | orb1937976 |
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Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB30172 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 71695 Da |
Target | This PALM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 546-574 amino acids from the C-terminal region of human PALM3. |
UniProt ID | A6NDB9 |
NCBI | NP_001138500.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Paralemmin-3, PALM3 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-PALM3 Antibody (C-term) at 1:1000 dilution. Lane 1: U-20S whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: SH-SY-5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 72 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
PALM3 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PALM3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PALM3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.