PAK2 Antibody

• Catalog Number: orb1239715
• Category: Antibodies
• Description: PAK2 Antibody
• Target: PAK2
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rabbit
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: PAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: PAK2 Antibody is affinity chromatography purified via peptide column.
• Immunogen: Anti-PAK2 antibody (orb1239715) was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PAK2. The immunogen is located within amino acids 20-70 of PAK2.
• UniProt ID: Q13177
• MW: Predicted: 58kDObserved: 58 kD
• Tested applications: ELISA, IF, IHC-P, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PAK2 Antibody: PAK65, PAKgammaGamma-PAK, PAK-2
• Note: For research use only
• NCBI: NP_002568

Western Blot Validation in Jurkat Lysate. Loading: 15 µg of lysates per lane. Antibodies: PAK2 orb1239715 (A: 0.5 µg/mL, B: 1 µg/mL and C: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PAK2 orb1239707 (1 µg/mL), PAK2 orb1239715 (1 µg/mL) and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PAK2 orb1239715 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PAK2 orb1239715 (1 µg/mL per lane), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of PAK2 in Mouse Spleen Tissue. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PAK2 antibody (orb1239715) at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of PAK2 in Mouse Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen tissue labeling PAK2 with orb1239715 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).