Paederosidic acid

SKU: orb1301536

Description

Paederosidic acid

Research Area

Cell Biology

Key Properties

• CAS Number: 18842-98-3
• MW: 464.44
• Purity: 99.96%
• Formula: C₁₈H₂₄O₁₂S
• SMILES: [H][C@]12[C@H](O[C@]3([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)OC=C(C(O)=O)[C@@]1([H])[C@@H](O)C=C2COC(=O)SC
• Target: Bcl-2 Family,Apoptosis
• Solubility: DMSO:55 mg/mL (118.42 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.31 mM)

Bioactivity

• In Vivo:
  Anticonvulsant and sedative effects of paederosidic acid isolated from Paederia s andens (Lour.) Merrill. In mice and rats.Paederosidic acid (5, 10, 20, and 40 mg/kg, ip) had significant anticonvulsant and sedative effects. paederosidic acid increased brain gamma-aminobutyric acid and decreased glutamic acid in the brain, and it up-regulated expressions of GAD 65. Paederosidic acid may be a promising future therapeutic agent for treatment of epileps.
• In Vitro:
  Paederosidic acid(PA) showed significant anti-tumor activity on lung cancer in vitro the mechanisms were involved in inducing mitochondria-mediated apoptosis via up-regulation of caspase-3, caspase-8, caspase-9, Bid, Bax, down-regulation of Bcl-2 and stimulatin the release of Cyto-C from mitochondria.JNK phosphorylation levels significantly increased concomitantly with decrease in Akt phosphorylation after treatment with PA in A549 cells. however, JNK siRNA-transfected cells diminished PA-induced caspase-3, 8 and 9, Bid and Bax activaton while enhance the Bcl-2 activation. PA-induced JNK activation played an important functional role in apoptosi.
• Cell Research:
  The anti Proliferative effects of PA on A549 cells were evaluated by MTT method an the IC50 Values were calculated. furthermore the PA-induced apoptosis in A549 cells was determined by fluorescence microscope via staining with DAPI and by flow cytometer via staining with FITC conjugated Annexin V/PI the expression of apoptosis-related or signaling proteins was investigated by Western blottin
• Animal Research:
  Anticonvulsant activities of paederosidic acid were evaluated by maximal electroshock and pentylenetetrazole-induced seizures in Male mice. Then, pentobarbital sodium-induced sleeping time and locomotor activity tests in mice were used to evaluat the sedative effects of paederosidic acid. Finally the mechanism of paederosidic acid was explored by evaluatin the contents of Glu and GABA in the brain, and Western blot was used to measure GAD65 expression in the mouse brai.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

acid, A549, Apoptosis, Bcl-2, cancer, Inhibitor, inhibit, Paederosidic, Paederosidic acid, Lung

Compound Identifiers

PubChem CID

21592249