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Catalog Number | orb1937871 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Target | This PABPN1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of human PABPN1L. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Mouse, Other, Rat |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
UniProt ID | A6NDY0 |
MW | 30386 Da |
Tested applications | FC, IF, IHC-P, WB |
Dilution range | IF: 1:10~50, WB: 1:500-1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Antibody Type | Primary Antibody |
Clone Number | RB29922 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Embryonic polyadenylate-binding protein 2, Embryon Read more... |
Note | For research use only |
NCBI | NP_001073956.2 |
All lanes: Anti-PABPN1L Antibody (Center) at 1:500-1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: human kidney lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of PABPN1L Antibody (Center) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
PABPN1L Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PABPN1L Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PABPN1L Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.