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Description
Images & Validation
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Item 1 of 1
| Tested Applications | ELISA, FC, ICC, IP, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however try:WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa)ICC/IF: Use at an assay dependent dilution. PubMed: 17977970Flow Cyt: Use 1µg for 106 cells |
| Reactivity | Gallus, Hamster, Human, Rabbit, Xenopus |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 10E10 |
| Immunogen | Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI |
| Target | PABP |
| Purification | Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS + 0.1% sodium azide; |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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