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Item 1 of 4
Item 1 of 4
| Catalog Number | orb769964 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to p70 S6 kinase alpha (phospho-Thr444) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr421. AA range:411-460 |
| MW | 70 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-RPS6KB1 antibody, anti-STK14A antibody, anti- Read more... |
| Note | For research use only |
Western blot analysis of LOVO using p-p70 S6 kinase α (T444) antibody. Antibody was diluted at 1:500.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p70 S6 Kinase (Phospho-Thr421) Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Thr421) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using p70 S6 Kinase (Phospho-Thr421) Antibody. The lane on the left is blocked with the phospho peptide.
