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Item 1 of 4
Item 1 of 4
| Catalog Number | orb764265 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to p70 S6 kinase alpha (phospho-Thr229) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Porcine, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr229. AA range:195-244 |
| MW | 60 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-RPS6KB1 antibody, anti-STK14A antibody, anti- Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hela cell. 1, p70 S6 kinase α (phospho Thr229) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 Monoclonal Antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Western Blot analysis of various cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500.
Western Blot analysis of LOVO cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500.
Immunohistochemistry analysis of paraffin-embedded human brain, using p70 S6 Kinase (Phospho-Thr229) Antibody. The picture on the right is blocked with the phospho peptide.
