p70 S6 kinase α (phospho Thr229) rabbit pAb

SKU: orb764265

Description

Rabbit polyclonal antibody to p70 S6 kinase alpha (phospho-Thr229)

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Porcine, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr229. AA range:195-244
• Molecular Weight: 60
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-RPS6KB1 antibody, anti-STK14A antibody, anti-Ribosomal protein S6 kinase beta-1 antibody, anti-S6K-beta-1 antibody, anti-S6K1 antibody, anti-70 kDa ribosomal protein S6 kinase 1 antibody, anti-P70S6K1 antibody, anti-p70-S6K 1 antibody, anti-Ribosomal protein S6 kinase I antibody, anti-Serine/threonine-protein kinase 14A antibody, anti-p70 antibody

Immunofluorescence analysis of Hela cell. 1, p70 S6 kinase α (phospho Thr229) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 Monoclonal Antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500.

Western Blot analysis of LOVO cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500.

Immunohistochemistry analysis of paraffin-embedded human brain, using p70 S6 Kinase (Phospho-Thr229) Antibody. The picture on the right is blocked with the phospho peptide.