RELA Antibody / p65 NF-kB

SKU: orb1151604

Description

Mouse monoclonal antibody to p65

Research Area

Epigenetics, Infectious Diseases, NF-kB, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: FACS, IF, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Optimal dilution of the RELA antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: PCRP-RELA-2B6
• Immunogen: Recombinant full-length human Transcription factor p65 protein was used as the immunogen for the RELA antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free RELA antibody (PCRP-RELA-2B6) as confirmation of integrity and purity.

Immunofluorescent staining of PFA-fixed human HeLa cells using RELA antibody (green, clone PCRP-RELA-2B6) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with RELA antibody (blue, clone PCRP-RELA-2B6) and isotype control (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using RELA antibody (clone PCRP-RELA-2B6). These results demonstrate the foremost specificity of the PCRP-RELA-2B6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

Q04206