p65 Antibody / NF-kB

• Catalog Number: orb1151604
• Category: Antibodies
• Description: Mouse monoclonal antibody to p65
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: Recombinant full-length human Transcription factor p65 protein was used as the immunogen for the RELA antibody.
• UniProt ID: Q04206
• Tested applications: FACS, IF, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the RELA antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PCRP-RELA-2B6
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the RELA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This RELA antibody is available for research use only.
• Note: For research use only

SDS-PAGE analysis of purified, BSA-free RELA antibody (PCRP-RELA-2B6) as confirmation of integrity and purity.

Immunofluorescent staining of PFA-fixed human HeLa cells using RELA antibody (green, clone PCRP-RELA-2B6) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with RELA antibody (blue, clone PCRP-RELA-2B6) and isotype control (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using RELA antibody (clone PCRP-RELA-2B6). These results demonstrate the foremost specificity of the PCRP-RELA-2B6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.