p57 Antibody [KP10]

• Catalog Number: orb1252595
• Category: Antibodies
• Description: p57 Antibody [KP10]
• Clonality: Monoclonal
• Clone Number: KP10
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG2b, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the p57 antibody.
• Antibody Type: Primary Antibody
• Concentration: 0.2 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CDKN1C
• UniProt ID: P49918
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CDKN1C Antibody: BWS, WBS, p57, BWCR, KIP2, p57Kip
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the p57 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with p57 antibody (KP10).

Western blot testing of human Jurkat cell lysate with p57 antibody (clone KP10).

SDS-PAGE Analysis of Purified, BSA-Free p57 Antibody (clone KP10). Confirmation of Integrity and Purity of the Antibody.