p53 (phospho Thr18) rabbit pAb

• Catalog Number: orb764263
• Category: Antibodies
• Description: Rabbit polyclonal antibody to p53 (phospho-Thr18)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Thr18. AA range:1-50
• MW: 46
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: IF: 1:50-200 WB 1:500-2000, IHC 1:50-300 IHC 1:50-300
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-TP53 antibody, anti-P53 antibody, anti-Cellul
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of rat-lung tissue. 1, p53 (phospho Thr18) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of rat-lung tissue. 1, p53 (phospho Thr18) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, p53 (phospho Thr18) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of various cells using Phospho-p53 (T18) Polyclonal Antibody diluted at 1:2000.