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| Catalog Number | orb770299 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to p53 (phospho-Ser366) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Ser366. AA range:331-380 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 48 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-TP53 antibody, anti-P53 antibody, anti-Cellul Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of HELA cells using Phospho-p53 (S366) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p53 (Phospho-Ser366) Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser366) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ug/ml 24h, using p53 (Phospho-Ser366) Antibody. The lane on the right is blocked with the phospho peptide.
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p53(Phospho-Ser366) rabbit pAb [orb771478]
ELISA, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl
