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Description
Research Area
Cancer Biology
Images & Validation
−
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500-1:1000, IHC-P: 1:50-1:200, IF: 1:50-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide, corresponding to amino acids 351-400 of Human p53. |
| Molecular Weight | ~53,43 kDa |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53
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Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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