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p53 (Di Methyl Lys370) rabbit pAb

Catalog Number: orb763960

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb763960
CategoryAntibodies
DescriptionRabbit polyclonal antibody to p53 (Di-Methyl-Lys370)
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Concentration1 mg/ml
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from human p53 around the di-methylation site of K370.
MW53
Tested applicationsELISA, IF, IHC-P, WB
Dilution rangeIF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-TP53 antibody, anti-P53 antibody, anti-Cellul
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NoteFor research use only
p53 (Di Methyl Lys370) rabbit pAb

Immunofluorescence analysis of human-breast-cancer tissue. 1, p53 (Di Methyl Lys370) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

p53 (Di Methyl Lys370) rabbit pAb

Immunofluorescence analysis of human-breast-cancer tissue. 1, p53 (Di Methyl Lys370) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

p53 (Di Methyl Lys370) rabbit pAb

Immunofluorescence analysis of human-liver-cancer tissue. 1, p53 (Di Methyl Lys370) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

p53 (Di Methyl Lys370) rabbit pAb

Immunofluorescence analysis of human-liver-cancer tissue. 1, p53 (Di Methyl Lys370) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.