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Catalog Number | orb763960 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to p53 (Di-Methyl-Lys370) |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Immunogen | Synthesized peptide derived from human p53 around the di-methylation site of K370. |
MW | 53 |
Tested applications | ELISA, IF, IHC-P, WB |
Dilution range | IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-TP53 antibody, anti-P53 antibody, anti-Cellul Read more... |
Note | For research use only |
Immunofluorescence analysis of human-breast-cancer tissue. 1, p53 (Di Methyl Lys370) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of human-breast-cancer tissue. 1, p53 (Di Methyl Lys370) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of human-liver-cancer tissue. 1, p53 (Di Methyl Lys370) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of human-liver-cancer tissue. 1, p53 (Di Methyl Lys370) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.