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p53 Antibody / TP53

Catalog Number: orb606661

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606661
CategoryAntibodies
DescriptionRecognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
ClonalityRecombinant
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenRecombinant full length human protein was used as the immunogen for the recombinant p53 antibody.
UniProt IDP04637
Tested applicationsIHC-P, WB
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application notesOptimal dilution of the recombinant p53 antibody should be determined by the researcher.
Antibody TypePrimary Antibody
Clone NumberrTP53/1739
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis recombinant p53 antibody is available for research use only.
NoteFor research use only
p53 Antibody / TP53

Western blot testing of human HeLa cell lysate with recombinant p53 antibody (clone rTP53/1739). Expetected molecular weight ~53 kDa.

p53 Antibody / TP53

IHC testing of FFPE human breast carcinoma stained with recombinant p53 antibody (clone rTP53/1739). Required HIER: boil tissue sections in 10mM Citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.

p53 Antibody / TP53

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using recombinant p53 antibody (clone rTP53/1739). These results demonstrate the foremost specificity of the rTP53/1739 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

p53 Antibody / TP53

SDS-PAGE analysis of purified, BSA-free recombinant p53 antibody (clone rTP53/1739) as confirmation of integrity and purity.

  • p53 Antibody / TP53 [orb750107]

    FACS,  IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg, 100 μg
  • p53 Antibody / TP53 [orb750106]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • p53 Antibody / TP53 [orb606469]

    IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg, 100 μg
  • p53 Antibody / TP53 N-Terminal Region [orb606470]

    IHC-P,  WB

    Human

    Mouse

    Recombinant

    Unconjugated

    20 μg, 100 μg
  • p53 Antibody / TP53 [orb639969]

    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg