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P4HA1 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:50-1:500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | prolyl 4-hydroxylase, alpha polypeptide I |
| Target | P4HA1 |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Disclaimer | For research use only |
Alternative Names
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PC-3 cells were subjected to SDS PAGE followed by western blot with orb395399 (P4HA1 antibody) at dilution of 1:1000.

Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using orb395399 (P4HA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb395399 (P4HA1 Antibody) at dilution of 1:500 heat mediated antigen retrieved with Tris-EDTA buffer (pH9).
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Protocol Information
P4HA1 Antibody (orb395399)
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