P45 NF-E2 Rabbit Polyclonal Antibody

SKU: orb559475

Description

P45 NF-E2 Rabbit Polyclonal Antibody

Research Area

DNA and RNA Research, Epigenetics and Nuclear Signaling, Transcription Factors

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NF-E2 (273-373/373aa)
• Target: NFE2
• Molecular Weight: 41 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NF-E2; NF-E2/P45; p45; p45NFE2; p45nf-e2; NFE2_HUMAN; NFE2; Leucine zipper protein NF-E2; Nuclear factor, erythroid-derived 2 45 kDa subunit; p45 NF-E2; NFE2_MOUSE; NFE2_RAT; nuclear factor, erythroid 2; nuclear factor (erythroid-derived 2), 45kD; nuclear factor (erythroid-derived 2), 45kDa

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p45 NF-E2) Polyclonal Antibody, Unconjugated (orb559475) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

NFE2

UniProt

Q16621