Recombinant deltaNp63 Antibody / p40

SKU: orb2636600

Description

p40, the deltaN form of p63, is thought to function as a stem cell factor, responsible for maintaining cells in an uncommitted state with regenerative potential a role that may be recapitulated in tumors derived from these cells. p40 is normally expressed in the basal or progenitor cell layer of stratified epithelia (eg. squamous, urothelial, bronchial), basal cells of some glandular epithelia (eg. prostate), as well as myoepithelial cells of breast and salivary glands, trophoblasts and thymic epithelial cells. In tumor tissues, p40 expression is specific for squamous cell carcinoma. p40 is equivalent to p63 in sensitivity for lung squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. p40 appears to be a more reliable marker for squamous cell carcinoma.

Images & Validation

• Tested Applications: ELISA, FACS, IHC-P
• Dilution Range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant deltaNp63 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: P40/4396R
• Immunogen: A portion of amino acids 1-100 was used as the immunogen for the recombinant deltaNp63 antibody.
• Purification: Protein A affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

FACS staining of PFA-fixed human HeLa cells with recombinant deltaNp63 antibody (blue, clone P40/4396R) and isotype control (red).

IHC staining of FFPE human prostate tissue with recombinant deltaNp63 antibody (clone P40/4396R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate tissue with recombinant deltaNp63 antibody (clone P40/4396R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

Q9H3D4