P2Y9 Polyclonal Antibody

SKU: orb1420914

Description

Rabbit polyclonal antibody to P2Y9.

Research Area

Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Disclaimer: For research use only

Alternative Names

Lysophosphatidic acid receptor 4, LPA receptor 4, LPA-4, G-protein coupled receptor 23, P2Y purinoceptor 9, P2Y9, Purinergic receptor 9, Lpar4, Gpr23, Lpa4, P2y9

Sample: Brain (mouse) Lysate at 40 ug. Primary: Anti- P2Y9 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 48 kD.

Sample: HL-60 Cell (Human) Lysate at 30 ug. Primary: Anti-P2Y9 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 55 kD. Observed band size: 55 kD.

Sample: k562 (human) cell Lysate at 40 ug. Primary: Anti- P2Y9 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 48 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug. Ovary (Mouse) Lysate at 40 ug. Primary: Anti-P2Y9 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 57 kD.

Sample: HL-60 (Human) Cell Lysate at 30 ug. K562 (Human) Cell Lysate at 30 ug. Primary: Anti-P2Y9 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 57 kD.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Quick Database Links

UniProt

Q8BLG2