P2Y9 Polyclonal Antibody

• Catalog Number: orb1420914
• Category: Antibodies
• Description: Rabbit polyclonal antibody to P2Y9.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Porcine, Rat
• UniProt ID: Q8BLG2
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Lysophosphatidic acid receptor 4, LPA receptor 4,
• Research Area: Epigenetics, GPCR, Neuroscience, Signaling Pathway
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Brain (mouse) Lysate at 40 ug. Primary: Anti- P2Y9 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 48 kD.

Sample: HL-60 Cell (Human) Lysate at 30 ug. Primary: Anti-P2Y9 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 55 kD. Observed band size: 55 kD.

Sample: k562 (human) cell Lysate at 40 ug. Primary: Anti- P2Y9 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 48 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug. Ovary (Mouse) Lysate at 40 ug. Primary: Anti-P2Y9 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 57 kD.

Sample: HL-60 (Human) Cell Lysate at 30 ug. K562 (Human) Cell Lysate at 30 ug. Primary: Anti-P2Y9 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 57 kD.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.